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The (His) 6 -tag was cleaved using overnight TEV digestion, followed by ion-exchange chromatography, and finally SEC using a Superdex 200 column (HiLoad 16/600, Cytiva)
FIGURE 3 5 Mechanistic differences of ferroptosis in normal and cancerous tissues Ferroptosis, an iron-dependent lipid peroxidation-driven form of cell death, exhibits significant differences in its mechanisms between normal and cancerous tissues (Jiang X
Hypercatabolism of IgG, IgA, IgM, and albumin in the Wiskott-Aldrich syndrome: anique disorder of serum protein metabolism
BPC157 was added at concentrations of 5, 10, 20, and 40 g ml-1 for the indicated periods
77 In a mouse model of endometriosis, the presence of ectopic endometrial lesions did not impact the number of mature MII oocytes per mouse but was associated with a lower proportion of normal oocytes (61 versus 83%) and incomplete extrusion or division of the first polar body and spindle abnormalities
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