The challenge is not simply eating fewer calories, but ensuring that the calories you do consume are nutrient-dense and support your body's essential functions
Diabetes 45 , 16381643 (1996)
The use of a biologically relevant substrate, which makes the results relevant to oxidative reactions in vivo, is this methods main advantage over other in vitro assays
What the researchers were discovering was that in the feces of these chronic laminitis horses was an abundance of a type of bacteria, which I believe at the time were lactic acid types, more specifically Streptococcus bovis
J., Van Rosmalen, J., Uyl-de Groot, C
Karakasis P, Patoulias D, Pamporis K, Stachteas P, Bougioukas KI, Klisic A, Fragakis N, Rizzo M