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After incubation with the primary antibody, the cells were washed with PBST and stained with secondary antibodies (Goat Anti-Rabbit IgG H&L Alexa Fluor 488, Abcam, ab150077

Recently, a hybrid model (cuprizone autoimmune encephalitis or CAE) combining the myelin-toxic effects of cuprizone (Zirngibl et al., 2022) together with an immune stimulation identical to EAE but without extrinsic myelin peptides, provided proof-of-principle for the notion that a primary biochemical dysmyelination can result in a very typical inflammatory demyelinating white matter lesion in an immune-stimulated host (Caprariello et al., 2018)

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A small, balanced snack can help stabilize energy levels and reduce discomfort

It all depends on the dose of free radicals youre exogenously exposed to (in other words, the accumulation of free radicals from external forces)