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All the data were subjected to analysis of variance (ANOVA) and means were then separated using the Least Significant Difference (LSD) using SAS (version 8.2

Using forceps, the stimulus was applied to the rats hind limb by momentarily pinching the areas innervated by the tibial, fibular, and sural nerves

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The sublingual delivery route administers MT-II to the mucosa beneath the tongue, where the dense sublingual vasculature allows for systemic peptide absorption without the hepatic first-pass metabolism that limits oral bioavailability in most peptide formulations