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J Exp Bot 65(5):12411257 Cairns NG, Pasternak M, Wachter A, Cobbett CS, Meyer AJ (2006) Maturation of Arabidopsis seeds is dependent on glutathione biosynthesis within the embryo

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Studies published in the 1990s showed that the structural changes in IGF1 LR3 resulted in dramatically reduced binding to IGFBPs while keeping high affinity for the IGF-1 receptor

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full result over 23 cycles (roughly 46 months )

Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)