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Following incubating with 2 M fura-2 AM (DOJINDO, Kumamoto, Japan) for 30 min at 37 C, the cells were mounted in chamber and superfused at 1.3 ml min 1 at 30 C with HKRB containing 5.6 mM glucose

A., Serraj, K., Vogel, T., & Kaltenbach, G

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This approach often prevents side effects by allowing your body to adjust to the medicine slowly