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Briefly, Huh7 cells were lysed in buffer (150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% NP-40, 9 g ml 1 leupeptin, 9 g ml 1 pepstatin, 10 g ml 1 chymostatin, 3 g ml 1 aprotinin, 1 mM PMSF and 100 U per ml RNase inhibitor) and centrifuged (12,000 g , 30 min, 4 C)

152154 In the classical pathway, hepatocellular SIRT1 deacetylates FOXO1 105 and FOXO3a, 155 inducing expression of autophagy-related proteins such as Atg5, Atg7, and Beclin-1, which promote autophagosome formation and maturation

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Alli AA, et al

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