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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

To start, you may need them every day or every other day

Factors such as your current health status, lifestyle, and specific goals should be taken into account to tailor a regimen that delivers maximum benefits

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The reconstituted solution should appear clear with a faint blue tint, which is characteristic of the copper(II) complex