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2.9 Assay of hepatic Nrf2 and NF-B gene expressions 2.9.1 RNA extraction The RNeasy QIAamp -Mini kit (Qiagen, Hilden, Germany) was used for the extraction of RNA from tissue samples after 30 mg of tissue was added to 600 L of RLT buffer containing 10 L of-mercaptoethanol per 1 mL

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After 24 h, the medium was replaced with fresh medium containing various concentrations of CM-EVs (150% v/v)

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Rev Endocr Metab Disord 26 , 679692 (2025)