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After incubation, SPA beads were pelleted by centrifugation (10 minutes, 1500 rpm) and counted in a TopCount (Perkin Elmer)

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Obesity and hypertension represent convergent public health challenges, as both contribute substantially to preventable cardiovascular disease and premature mortality

To remove the cleaved tag and TEV protease, the dialysed protein was loaded onto an equilibrated 5-mL HisTrap HP column, and the flowthrough was collected