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If an REH chooses to report the Emergency Care Access & Timeliness eCQM, the REH would report the data using the proposed methods and standards specified in section XVI.C.2

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In this case, store the vials in a cool, dry environment and keep them away from excessive heat, humidity, and direct light

The reaction parameters were the following: enzyme activation and denaturation at 95C/3 min, amplification at 95C/03 sec, annealing at 60C/1 min, 45 cycles

Keep the tubes at 28C, away from light, and use them before the expiry date for reliable results