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Erythrocytes were lysed twice by adding DEPC-treated H 2 O and NaCl (8.8%), followed by centrifugation (450 x g, 6 min, 4C, Universal 320 R, Hettich, Tuttlingen, Germany)

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Mol Cell Biol (2000) 20(10):338795.10.1128/MCB.20.10.3387-3395.2000 80 SaharinenPSilvennoinenO

Although individuals vary in both specificity and timing of anti-HCV seroconversion, HCV-specific antibodies are generally detectable 7 to 31 weeks after infection