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Epac fl/fl mice were crossed with Braf V600E/+ /Pten fl/fl ( BPT ) mice [13] to generate double heterozygous BPT/Epac1 +/ fl /Epac2 +/fl , double homozygous ( BPT/Epac1 fl/fl /Epac2 fl/fl ) , Epac1 fl/fl ( BPT/Epac1 fl/fl ) and Epac2 fl/fl (BPT/Epac2 fl/fl ) as described previously

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Studies find that glutathione deficiency is associated with higher oxidative stress and increased risk of chronic diseases

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