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A 2020 review in Journal of Physiology and Pharmacology called for multi-center, GLP-compliant studies to verify the Zagreb group's findings 15

Restivo et al

Loren Pickart in 1973, GHK-Cu was discovered when Pickart observed that albumin from young donors (age 20-25) could stimulate aged liver tissue to synthesize proteins at a youthful rate, and the active factor was traced to this small copper peptide

Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

10.1038/aps.2015.16 14 MotaA