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Preparation of stock solution of n-acetyl l-cysteine 10 mg of n-acetyl l-cysteine was accurately weighed, transferred into a 10-ml volumetric flask and dissolved using a small amount of 0.1 N KOH, and then, the volume was made up using distilled water

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The voltage specified on a LiPo battery is the total value of the Nominal voltage of each cell

Compared with saline, DREADD-based activation of LS GLP-1R neurons via CNO reduced a 2-hour consumption period of both standard chow and high-sucrose food during the dark period when mice normally consume food actively (Figure 5, C and D)

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In further experiments, we initially added rolipram (100 nM) to the organ bath