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Of note, the pulsatile activity greatly varies between individuals (Appendix Fig S3A)

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The method of any one of 29

Conn HO, Leevy CM, Vlahcevic ZR, Rodgers JB, Maddrey WC, Seeff L, Levy LL

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Cells were first gently lysed with the cytoplasmic extraction buffer to release cytoplasmic components, and the supernatant was collected after centrifugation as the cytoplasmic protein fraction