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Vitamin C (Liposomal) 500mcg

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10.1186/1752-1947-2-146 J Med Case Rep

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The cell growth medium consisted of 50% Keratinocyte Serum-Free Medium (Gibco, USA), 33.75% Dulbeccos modified Eagle medium (DMEM, Gibco, USA), 11.25% Hams F-12 Nutrient Mixture (Gibco, USA), 5% fetal bovine serum (FBS, Gibco, USA), 5 ng/mL epidermal growth factor (EGF, Gibco, USA), 50 ng/mL bovine pituitary extract (Scienceu, USA), 0.4 g/mL hydrocortisone (Sigma, USA), 5 g/mL transferrin (Sigma, USA), 5 ng/mL bovine insulin (Sigma, USA), 0.18 mM adenine (Sigma, USA), 2 nM 3,3,5-triiodo-L-thyronine (Sigma, USA), 100 units/mL penicillin (Gibco, USA), and 100 g/mL streptomycin (Gibco, USA)