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Prior to permeability experiments, cells were washed with transport buffer (0.12 M NaCl, 25 mM NaHCO 3 , 3 mM KCl, 2 mM MgSO 4 , 2 mM CaCl 2 , 0.4 mM K 2 HPO 4 , 1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and 0.1% human platelet poor derived serum) and 100 L of the molecule of interest in transport buffer was introduced into the apical well

doi: 10.31491/apt.2020.03.014

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