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The cover slip was slowly removed and the slides were lysed in a solution containing 100 mM EDTA, 2.5 M NaCl, 0.2 M NaOH, and 10 mM Tris (pH 10) with freshly prepared 1% Triton x-100 for 40 min

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The Biochemical Journal, 361 (Pt 3), 417429

Consistent nutrient availability supports sustained anagen activity and reduces premature transition into telogen