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doi: 10.1016/S2213-8587(21)00203-5

Cells were seeded at 2.53 10 6 viable cells per ml and transfected with 0.8 mg/ml DNA construct using the Expifectamine TM 293 transfection reagent (ThermoFisher Scientific)

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administered into the rats fasted 16 h, and at 10:00 standard chow was given and food intake was measured

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