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In vitro assays using BaF-BO3 cells transfected with the hGH receptor confirmed that AOD9604 does not compete for the hGH receptor and does not induce cell proliferation unlike intact hGH establishing that it operates via a novel mechanism distinct from traditional hGH-stimulated pathways

Akin et al

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L-Glutamine: A vital amino acid

Then samples are analyzed by tandem mass spectrometry (NEM was used as masking agent and added to collecting tubes) and the analytes are quantified using the stable-isotope labelled analogs