2.7 Fixation, staining and counting of clonogenic assay For SW837 cells, a volume of 500 L fixing/staining solution (0.05% (w/v) crystal violet (Sigma), 25% (v/v) Methanol (Sigma) in PBS) was added to each well and incubated for 30 min, at RT
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Inflammation correlates with symptoms in chronic fatigue syndrome
Consume after meals with water
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Because traditional centrifugation-based methods are too slow to preserve ER metabolite content, we adapted an immunopurification strategy previously developed for other organelles 1315