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Cell culture and drug treatment The human liver cancer cell line HepG2 was obtained from ATCC and was cultured in DMEM glutamax high glucose medium (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/mL penicillin/streptomycin (Invitrogen), in a humidified incubator at 37C and 5% CO 2 atmosphere

Duration: Treatment typically lasts 3-12 months, depending on individual goals and response

doi:10.1016/j.bmc.2008.10.006 41

S., Alkass, N., Andrade, M

These compounds increase endogenous growth hormone release, which supports fat loss, muscle preservation, sleep quality, and recovery without the side effects of exogenous growth hormone or the suppression caused by anabolic steroids

ATP exposure stimulates glutathione efflux as a necessary switch for NLRP3 inflammasome activation