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The carnitine bound OCTN2-occluded structure defines the substrate and sodium ion binding sites In the presence of 10 mM l-carnitine the structure of OCTN2 was determined in the fully occluded, carnitine- and Na + -bound conformation (Fig

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Size exclusion chromatography Gel filtration experiments were carried out on a Superdex 200 10/300 GL column (manufacturer's exclusion limit 600 kDa for proteins) on an KTA-FPLC (GE HealthCare Biosciences)

For example, if you're adding 1 mL of water, pull the plunger back to the 1 mL mark