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Here, we report that the triagonist IUB447 did not enhance cAMP accumulation in pancreatic islets and blocking of the adenylate cyclase had a minimal impact on triagonist-induced insulin secretion

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In another broad aspect the present invention relates to an anion exchange chromatography process for purifying a peptide from a mixture comprising said peptide and related impurities, comprising the step of: separating said peptide and said related impurities of said mixture by elution in a solution consisting essentially of an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said peptide has a negative local or overall net charge different from the local or overall negative net charge of said related impurities so as to remove said related impurities

doi: 10.1186/s12864-017-4195-3 48 GuoD.ParkC.LiY.LiB.YangQ.DengY.et al

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