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The spleen was carefully lifted to expose the pancreas, and 50 L of cell suspension (1 10 6 cells) was slowly injected into the pancreatic parenchyma using an insulin syringe with a needle depth of approximately 3 mm

This mechanistic clarity makes Semaglutide the appropriate reference compound for establishing baseline GLP-1 pathway effects before investigating what dual or triple agonism adds

Dispose of the syringe and needle in a Sharps Container

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