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Cell culture Bone marrow-derived macrophages (BMDMs) were harvested from the femur and tibia of C57BL/6J mice and cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 10% L929 cell-conditioned medium, and maintained in a humidified incubator with 5% CO 2 at 37 C for 7 days [36]
[14] Review literature reports that endogenous plasma GHK falls from roughly 200 ng/mL around age 20 to under 80 ng/mL by age 60
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For instance, while topical agents may suffice for localized mild disease, systemic therapies are often warranted for extensive or refractory cases (Rendon & Schakel 2019)
