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TrxR1 Activity Assays TrxR1 activity was assessed using 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) (D8130-5G, Sigma) also known as Ellmans reagent to detect the rate and level of reductive activity from TrxR1

Superposition of the holo-structure and the GSSG-structure (RMSD 0.28) reveals that the Phe13 residue occupies the same position as that found in the holo structure (Fig

Therefore, it is important to note that falsifications without an API can be expected