3.2 Engineering RBC metabolism and storage conditions During their storage at refrigerated temperatures, red blood cells go through several biochemical and morphological modifications known as storage lesion. Indeed, during this period, 2,3-BPG and other high-energy phosphate compounds gradually decrease because of the reduced glycolytic activity at 1C6C storage temperatures, the acidification of the intracellular pH, and the reversible and irreversible oxidation of the active site of rate-limiting glycolytic enzymes, such as glyceraldehyde 3-phosphate dehydrogenase
Cells were incubated with 1 M CellRox DeepRed at 37 C for 1 h and detached by trypsination
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Link Bottini, G., et al
Operation Equipment: Spectrophotometer/microplate reader Catalog Number: BC1185 Size: 100T/96S Components: Components: Reagent I:100 mL1
Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers
