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The results demonstrated that, compared with the normoxia group, hypoxia significantly enhanced the expression of key genes in the HIF-1 signaling pathway ( ENO2 , HK2 , SLC2A1 , and PFKP ), and inhibited the expression of genes associated with the Hippo signaling pathway ( WTIP , CCN2 , NF2 , and AXIN2 ) (Figure 6e)

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The primary antibodies used were against ILF3 (ab92355, Abcam, UK), SLC7A11 (ab307601, Abcam, UK), GPX4 (ab252833, Abcam, UK), GAPDH (ABclonal, A19056), PCNA (ab92552, Abcam, UK), HDAC1 (A19571, ABclonal, China), HDAC2 (A19626, ABclonal, China), HDAC6 (A3572, ABclonal, China), DEPTOR (A9447, ABclonal, China), mTOR (2972S, Cell Signaling Technology, USA), p-mTOR (5536S, Cell Signaling Technology, USA), H3K14ac (A7254, ABclonal, China), PD-L1 (66248-1-Ig, Proteintech, China), CD8 (66868-1-Ig, Proteintech, China), pan acetyl lysine (PTM-105, PTM Bio, China), and 4-hydroxynonenal (4-HNE) (bs-6313R, Bioss, China)

En investigacin se combinan porque uno favorece la formacin de vasos nuevos y el otro la migracin de clulas al sitio de la lesin

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Through inflammatory cell infiltration (neutrophils, T/B cells), cytokine network imbalance, and vascular wall structural destruction, it ultimately leads to vascular stenosis, occlusion, or rupture, resulting in tissue and organ ischemia or hemorrhage (210, 211)