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Participants in each cohort were randomized 1:1 to receive either 2 g elemental oral LC supplementation or placebo daily for 21 days

Cells were grown at 30C in Hartwells complete (HC) medium with 2% glucose and lacking leucine and uracil (-Leu-Ura) to ensure retention of both plasmids to an OD 6 00 of 3.0

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