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The homogenized lysate was then run through QIAshredder columns (Qiagen, 79656)

3.2 Study data 3.2.1 Studies with a control group One controlled study comparing polysomnography (PSG) data of children with GHD with age-matched controls found that children with GHD had significantly lower total sleep time, sleep efficiency, movement time, and time spent in rapid eye movement (REM) sleep and stage 2 non-REM sleep, plus a general decrease in electroencephalogram (EEG) arousability ( In another controlled study, a group of seven children with GHD underwent PSG before and after 12 weeks of GH therapy ( The third controlled study included five children with GHD and 10 healthy controls ( 3.2.2 Studies without a control group In a study evaluating GH secretion during sleep in children with short stature, peaks of GH secretion were found to occur primarily during slow-wave and deep sleep ( There were several inconsistencies in the findings of the identified studies

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