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PMID: 197 ChengWYangJPanYQuHDuanZWuJet al

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For the concentration-dependent responses of ghrelin or ibutamoren, 50 L HBSS buffer containing different concentrations of ligand was injected to each well, and fluorescence was measured constantly in real time for 90 s

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Immediate & Consistent Cold Storage: As soon as the peptide is dissolved, place it in the refrigerator

For mitochondria analysis, cells were washed twice in RPMI media (no FBS) loaded with 10 nmol ml 1 Mitotracker Green (Thermo Fisher Scientific), 25 nmol ml 1 TMRM (Thermo Fisher Scientific), or 5 mmol ml 1 MitoSox Red for 20 min at 37 C in RPMI (no FBS)