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Our clinicians can help build a personalized injection plan

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Remember, theres quite a bit of flexibility here so if you need to use a buffer besides PBS to accommodate your interacting protein, just use that one for the binding buffer as well

( it becomes blunt when it touches the glass- ouch!) Use a long needle to withdraw ( 1 1/2 inch ) Then change to injection needle

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker