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Immunofluorescence Staining and Quantification PBS-washed cells on coverslips were fixed with 4% paraformaldehyde (15 min, RT) and permeabilized with 0.1% Triton X-100 (5 min, RT)
Key Research Characteristics: Extended half-life (~2030 hours vs ~1215 minutes for native IGF-1) due to reduced IGFBP binding Potent agonist at IGF-1R, studied for roles in cellular proliferation, differentiation, and survival signalling Investigated in models of muscle hypertrophy, neuroprotection, and metabolic regulation 83 amino acid analogue
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This review describes our current understanding of the genetic contributions to psoriasis and PsA and the techniques used in determining these contributions
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