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Following a further 30-min incubation, the sample was buffer-exchanged four times with the same sodium phosphate buffer

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Mol Cell Biochem 39:357368 Thon VJ, Vigneron-Lesens C, Marianne-Pepin T, Montreuil J, Decq A, Rachez C, Ball SG, Cannon JF (1992) Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae