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PA-TU-8902, MIA PaCa-2, HEK293T and U2OS cells were cultured in Dulbeccos Modified Eagles medium (DMEM, P04-03550, PAN Biotech) supplemented with 10% FBS, 1% NEAA and 1 mM sodium pyruvate (P04-43100, PAN Biotech)

Advantages of Glutathione: It is the preformed, active compound

The more phenolic hydroxyl groups there are, the more sites can be oxidized and the stronger the ability to scavenge oxygen free radicals (Table 2)

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A total 2 10 5 cells were used in each experiment for detection of total glutathione and 10 6 cells for GSSG measurement

If you are dealing with melasma or pigmentation, it is a useful strategy to combine both agents