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Kawahara T, Suzuki G, Mizuno S, Inazu T, Kasagi F, Kawahara C, et al
For safety, the calculator wont recommend a target below 1,200 kcal a day for women or 1,500 kcal a day for men
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NCTC1469 cells, a normal mouse hepatocyte cell line, were obtained from the American Type Culture Collection and were cultured in DMEM containing 10% (v/v) fetal horse serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
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