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At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)

Cardiovasc Res 75:2208, PMID: 17451659 Giustarini D, Rossi R, Milzani A, Colombo R, Dalle-Donne I (2004) S -glutathionylation: from redox regulation of protein functions to human diseases

We used 7-day-old mice randomly assigned to hypoxic or control groups

It is meant to protect against tissue damage and promote healing

Patients should be carefully monitored and any physical changes be recorded

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