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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

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Squeeze the entire contents of the packet into 13 oz

Angiotensin II (Ang II), a key component of RAAS, is known to stimulate the production of ROS, particularly in vascular smooth muscle cells and endothelial cells [80]

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