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Briefly, quantification of signal bias was performed using pharmacologically derived parameters of agonist affinity (Ka) and efficacy (tau) for each ligand in each of the three signalling pathways (cAMP accumulation, ERK1/2 phosphorylation, Intracellular Ca 2+ mobilisation)

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DU has served as a consultant for Novo Nordisk Inc

The cells were washed with cold PBS and were directly observed using a fluorescence microscope, or were digested and collected

One area of interest that has emerged is the role of glutathione in combating COVID-19