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Furthermore, the tumor suppressor p53 serves as a critical node connecting these pathways as it can promote ferroptosis via SLC7A11 repression while simultaneously regulating apoptotic gene expression (144)
The ligand-bound VDR heterodimerizes with the Retinoid X Receptor (RXR), and this complex recruits co-regulatory proteins to Vitamin D Response Elements (VDREs) in target genes ( Figure 2 VDR itself, or PDIA3, triggering intracellular cascades such as MAPK/ERK and PI3K/Akt
Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added
10.1016/j.bbr.2011.03.003 Behav Brain Res
Collection of lenses and preparation of homogenate The cataractous lenses were removed surgically by PECCE+IOL (Planned extra capsular cataract extraction+ intraocular lens) technique
