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Peptides were eluted on-line to an analytical column (Acclaim Pepmap RSLC C18, 75 m 75 cm)

CJC-1295 No DAC CJC-1295 No DAC Peptide available in 5 mg and 10 mg options

Chang SH, Barbosa-Tessmann I, Chen C, Kilberg MS, Agarwal A

Materials You Will Need Before beginning reconstitution of either BPC-157 or TB-500, ensure you have: Lyophilized peptide vial (verify identity, lot number, and purity certificate prior to opening) Appropriate solvent (see solvent selection section below) Sterile 1 mL or 3 mL syringe with a low-dead-volume tip Sterile needle (typically 23-25 gauge for vial penetration) Alcohol swabs A laminar flow hood or biosafety cabinet for sterile preparation Aliquot vials (low-binding microcentrifuge tubes or glass vials) if preparing multiple aliquots Labeling materials: lot number, concentration, date of reconstitution, initials Solvent Selection: BPC-157 vs TB-500 BPC-157 Solvent Options BPC-157 is highly water-soluble and reconstitutes readily in aqueous solvents

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