Then, the blots were incubated with the following antibodies overnight at 4 C: mouse anti-GLP-1R (1:200, Santa Cruz, CA, USA), mouse anti-GLP-1 (1:100, Santa Cruz, CA, USA), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), mouse anti-c-fos (1:200, Santa Cruz, CA, USA), rabbit anti-Iba-1 (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-PI3K (1:1000, Proteintech, China), rabbit anti-Akt (1:1000, Proteintech, China), rabbit anti-phospho-Akt (1:2000, Cell Signalling, Boston, MA, USA), rabbit anti-IL-1 (1:800, Wanleibio, China), rabbit anti-TNF- (1:800, Wanleibio, China), and mouse anti--actin (1:1000, Beyotime, China)
The peptide bonds holding the molecule together can break under vigorous agitation
Tirzepatide targets both GLP-1 and GIP receptors, making it more powerful for patients whose FTO rs9939609 or GIPR rs1800437 variants predispose them to appetite dysregulation
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