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The filtrate was diluted 1:500 with 50 mM phosphate-buffered saline, and the diluted liver homogenate was used for subsequent GPx activity assays

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Peptides were subjected to nanoelectrospray ionisation tandem mass spectrometry through the TripleTOF 5600 coupled in line to the HPLC system, with an electrospray voltage of 2.2 kV and capillary temperature of 270 C

Frontiers in Public Health , 8, 550753.