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Add solvent slowly Using a calibrated micropipette, add the calculated volume of solvent gently against the side of the vial not directly onto the lyophilized cake

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4.1.4 Antioxidant minerals: selenium and iodine Selenium contributes to antioxidant capacity through its role in selenoproteins such as glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) (87, 88)