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A rationally designed monomeric peptide triagonist corrects obesity and diabetes in rodents

doi: 10.1038/nri3608 181 GuKWuAYuBZhangTLaiXChenJet al

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They must not be used for clinical, diagnostic, or therapeutic applications

with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)