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This removes all ambient moisture, maximizing the compounds structural integrity and shelf life

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The enzymatic oxidation is catalyzed by a family of enzymes, such as cytochrome P450 (CYP), cyclo-oxygenase-2 (COX-2), peroxidase, tyrosinase (monophenol oxygenase), xanthine oxidase (XO), monoamine oxidase (MAO), and polyphenol oxidases (PPO), and is a reversible reaction using reducing agents such as H 2 or H 2 S [164]

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Methylcobalamin is an active form of Vitamin B12, a vital nutrient crucial for numerous metabolic processes and essential for supporting key bodily functions

Acetonitrile (HPLC-grade), triethylamine, formic acid, trichloroacetic acid (TCA), 2-mercaptoethanol, and N -ethylmaleimide (NEM) were obtained from Nacalai Tesque (Kyoto, Japan)