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Using unsupervised clustering of Ca 2+ genes, we observed segregation corresponding to sensitivity (Supplementary Fig

Mature 3T3-L1 adipocytes were washed with DMEM twice and then incubated in DMEM supplemented with 0.2% endotoxin and FA-free BSA for 48 h

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It comes in a multidose 30 mL vial (plastic, fliptop) from which you can withdraw multiple aliquots

The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume

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