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Yin XY, Cheng H, Wang WJ, Wang WJ, Fu HY, Liu LH, et al

The increased DHA uptake in mutant cells produced oxidative stress, increasing the level of reactive oxygen species (ROS) in cells because intracellular DHA was rapidly reduced back to vitamin C at the expense of glutathione (GSH), a master antioxidant in cells

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(6) Analyze the target protein by SDS-PAGE, along with fractions collected from different steps if necessary 4

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